| 质粒名称: | pMIG-hNFATc2/C - AVITEV |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74058 |
| 存储实验室: | Ria Baumgrass |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCV-IRES-GFP (pMIG) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7200 bp |
| 完整质粒大小: | 9935 bp |
| 载体修饰: | added SV40 ori |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | GFP |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | human NFATc2, isoform C,NFAT1,nuclear factor of activated T-cells 2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2700 bp |
| 基因突变: | NM 173091.3序列中的沉默突变A1723C（A1503C在载体ORF中的位置） |
| GenBank编号 | NM_173091.3 Gene-ID: 4773 |
| 启动子 | pMSCV-LTRs |
| 标签/融合蛋白: | AVI-TEV（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SacII(未破坏) |
| 3' 克隆位点: | SnaB1, Sgf1(未破坏) |
| 5' 测序引物 | CGTTCGACCCCGCCTCGATCC |
| 3' 测序引物 | AGCTTGATATCGAATTCCG |
|
文献引用方法 |
|
| 材料和方法部分: | pMIG-hNFATc2/C - AVITEV was a gift from Ria Baumgrass (Addgene plasmid # 74058 ; http://n2t.net/addgene:74058 ; RRID:Addgene_74058) |
| 参考文献部分: | Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326. 10.1074/jbc.M116.739326 PubMed 27637333 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMIG-hNFATc2/C - AVITEV 9935 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION retroviral expression plasmid for human NFATc2/C with C-terminal
BirA-biotinylation signal and TEV celavage site .
ACCESSION .
VERSION .
KEYWORDS pMIG-hNFATc2/C - AVITEV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9935)
AUTHORS Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M,
Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel
H, Radbruch A, Krause E, Baumgrass R
TITLE Identification of Novel Nuclear Factor of Activated T Cells
(NFAT)-Associated Proteins in T cells.
JOURNAL J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
PUBMED 27637333
REFERENCE 2 (bases 1 to 9935)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9935
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 43..560
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 624..965
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 1032..1448
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1378..1400
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1416..1432
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 3651..3660
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4266..4286
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 4299..4343
/codon_start=1
/product="peptide tag that allows for enzymatic
biotinylation"
/label=AviTag(TM)
/translation="GLNDIFEAQKIEWHE"
primer_bind 4376..4392
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 4377..4393
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 4428..4980
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4572..4589)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4799..4818
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4992..5711
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5037..5058)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5298..5317)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5645..5666
/label=EGFP-C
/note="EGFP, forward primer"
LTR 5802..6317
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(6486..6502)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6486..6502)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6499..6521)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6510..6526
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6534..6564)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6579..6600
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6717..6734)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6888..7476)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6968..6987)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7647..8507)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8270..8289
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8508..8612)
/gene="bla"
/label=AmpR promoter
primer_bind 8680..8698
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8736..8758)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 8858..8877
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(8900..8920)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8905..9234
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9085..9220
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9147..9166
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9282..9329
/label=EM7 promoter
/note="synthetic bacterial promoter "
primer_bind 9523..9545
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9537..9554
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 9538..9554
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9757..9776
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
1441 taggcgccgg aattagatct gcggccgcgg atttaaatcc caccatgaac gcccccgagc
1501 ggcagcccca acccgacggc ggggacgccc caggccacga gcctgggggc agcccccaag
1561 acgagcttga cttctccatc ctcttcgact atgagtattt gaatccgaac gaagaagagc
1621 cgaatgcaca taaggtcgcc agcccaccct ccggacccgc ataccccgat gatgtcctgg
1681 actatggcct caagccatac agcccccttg ctagtctctc tggcgagccc cccggccgat
1741 tcggagagcc ggatagggta gggccgcaga agtttctgag cgcggccaag ccagcagggg
1801 cctcgggcct gagccctcgg atcgagatca ctccgtccca cgaactgatc caggcagtgg
1861 ggcccctccg catgagagac gcgggcctcc tggtggagca gccgcccctg gccggggtgg
1921 ccgccagccc gaggttcacc ctgcccgtgc ccggcttcga gggctaccgc gagccgcttt
1981 gcttgagccc cgctagcagc ggctcctctg ccagcttcat ttctgacacc ttctccccct
2041 acacctcgcc ctgcgtctcg cccaataacg gcgggcccga cgacctgtgt ccgcagtttc
2101 aaaacatccc tgctcattat tcccccagaa cctcgccaat aatgtcacct cgaaccagcc
2161 tcgccgagga cagctgcctg ggccgccact cgcccgtgcc ccgtccggcc tcccgctcct
2221 catcgcctgg tgccaagcgg aggcattcgt gcgccgaggc cttggttgcc ctgccgcccg
2281 gagcctcacc ccagcgctcc cggagcccct cgccgcagcc ctcatctcac gtggcacccc
2341 aggaccacgg ctccccggct gggtaccccc ctgtggctgg ctctgccgtg atcatggatg
2401 ccctgaacag cctcgccacg gactcgcctt gtgggatccc ccccaagatg tggaagacca
2461 gccctgaccc ctcgccggtg tctgccgccc catccaaggc cggcctgcct cgccacatct
2521 acccggccgt ggagttcctg gggccctgcg agcagggcga gaggagaaac tcggctccag
2581 aatccatcct gctggttccg cccacttggc ccaagccgct ggtgcctgcc attcccatct
2641 gcagcatccc agtgactgca tccctccctc cacttgagtg gccgctgtcc agtcagtcag
2701 gctcttacga gctgcggatc gaggtgcagc ccaagccaca tcaccgggcc cactatgaga
2761 cagaaggcag ccgaggggct gtcaaagctc caactggagg ccaccctgtg gttcagctcc
2821 atggctacat ggaaaacaag cctctgggac ttcagatctt cattgggaca gctgatgagc
2881 ggatccttaa gccgcacgcc ttctaccagg tgcaccgaat cacggggaaa actgtcacca
2941 ccaccagcta tgagaagata gtgggcaaca ccaaagtcct ggagatcccc ttggagccca
3001 aaaacaacat gagggcaacc atcgactgtg cggggatctt gaagcttaga aacgccgaca
3061 ttgagctgcg gaaaggcgag acggacattg gaagaaagaa cacgcgggtg agactggttt
3121 tccgagttca catcccagag tccagtggca gaatcgtctc tttacagact gcatctaacc
3181 ccatcgagtg ctcccagcga tctgctcacg agctgcccat ggttgaaaga caagacacag
3241 acagctgcct ggtctatggc ggccagcaaa tgatcctcac ggggcagaac tttacatccg
3301 agtccaaagt tgtgtttact gagaagacca cagatggaca gcaaatttgg gagatggaag
3361 ccacggtgga taaggacaag agccagccca acatgctttt tgttgagatc cctgaatatc
3421 ggaacaagca tatccgcaca cctgtaaaag tgaacttcta cgtcatcaat gggaagagaa
3481 aacgaagtca gcctcagcac tttacctacc acccagtccc agccatcaag acggagccca
3541 cggatgaata tgaccccact ctgatctgca gccccaccca tggaggcctg gggagccagc
3601 cttactaccc ccagcacccg atggtggccg agtccccctc ctgcctcgtg gccaccatgg
3661 ctccctgcca gcagttccgc acggggctct catcccctga cgcccgctac cagcaacaga
3721 acccagcggc cgtactctac cagcggagca agagcctgag ccccagcctg ctgggctatc
3781 agcagccggc cctcatggcc gccccgctgt cccttgcgga cgctcaccgc tctgtgctgg
3841 tgcacgccgg ctcccagggc cagagctcag ccctgctcca cccctctccg accaaccagc
3901 aggcctcgcc tgtgatccac tactcaccca ccaaccagca gctgcgctgc ggaagccacc
3961 aggagttcca gcacatcatg tactgcgaga atttcgcacc aggcaccacc agacctggcc
4021 cgcccccggt cagtcaaggt cagaggctga gcccgggttc ctaccccaca gtcattcagc
4081 agcagaatgc cacgagccaa agagccgcca aaaacggacc cccggtcagt gaccaaaagg
4141 aagtattacc tgcgggggtg accattaaac aggagcagaa cttggaccag acctacttgg
4201 atgatgttaa tgaaattatc aggaaggagt tttcaggacc tcctgccaga aatcagacgt
4261 acgtagagaa cctgtacttc cagggcgtta acggcggtgg cctgaacgat atcttcgagg
4321 cacagaaaat cgaatggcat gaggttaact gagcgatcgc ttataagcgg ccgcgtcgag
4381 gtcgacggta tcgataagct tgatatcgaa ttccgccccc ccccctaacg ttactggccg
4441 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
4501 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
4561 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
4621 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
4681 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
4741 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
4801 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
4861 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
4921 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
4981 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
5041 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg
5101 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
5161 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
5221 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
5281 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
5341 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
5401 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
5461 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
5521 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
5581 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
5641 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
5701 tgtacaagta agaattcctg cagcccgggg gatccgtcga cctgcagcca agcttatcga
5761 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
5821 ggtttggcaa gctagcttaa ttaacgccat tttgcaaggc atggaaaata cataactgag
5881 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
5941 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
6001 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
6061 ctgaaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
6121 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
6181 tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
6241 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
6301 cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta
6361 ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca
6421 atactcctga aatagttcat tatggacagc gcagaagagc tggggagaat taattcgtaa
6481 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
6541 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
6601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
6661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
6721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
6781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
6841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
6901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
6961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
7021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
7081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
7141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
7201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
7261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
7321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
7381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
7441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
7501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
7561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
7621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
7681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
7741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
7801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
7861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
7921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
7981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
8041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
8101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
8161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
8221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
8281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
8341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
8401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
8461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
8521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
8581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
8641 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
8701 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
8761 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
8821 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
8881 agagtgcacc atatgctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
8941 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
9001 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
9061 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
9121 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
9181 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
9241 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
9301 catagtatat cggcatagta taatacgaca aggtgaggaa ccatatgcgg tgtgaaatac
9361 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
9421 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
9481 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
9541 aaacgacggc cagtgccaag ctcgctctcc cttatgcgac tcctgcatta ggaagcagcc
9601 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
9661 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
9721 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
9781 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
9841 ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa
9901 tatcaccagc tgaagcctat agagtacgag ccata
//
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